Mechanism of microtubule stabilization by taccalonolide AJ - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2017

Mechanism of microtubule stabilization by taccalonolide AJ

Résumé

As a major component of the cytoskeleton, microtubules consist of αβ-tubulin heterodimers and have been recognized as attractive targets for cancer chemotherapy. Microtubule-stabilizing agents (MSAs) promote polymerization of tubulin and stabilize the polymer, preventing depolymerization. The molecular mechanisms by which MSAs stabilize microtubules remain elusive. Here we report a 2.05 Å crystal structure of tubulin complexed with taccalonolide AJ, a newly identified taxane-site MSA. Taccalonolide AJ covalently binds to β-tubulin D226. On AJ binding, the M-loop undergoes a conformational shift to facilitate tubulin polymerization. In this tubulin–AJ complex, the E-site of tubulin is occupied by GTP rather than GDP. Biochemical analyses confirm that AJ inhibits the hydrolysis of the E-site GTP. Thus, we propose that the β-tubulin E-site is locked into a GTP-preferred status by AJ binding. Our results provide experimental evidence for the connection between MSA binding and tubulin nucleotide state, and will help design new MSAs to overcome taxane resistance.
Fichier principal
Vignette du fichier
ncomms15787.pdf (2.09 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

hal-02104497 , version 1 (25-01-2024)

Identifiants

Citer

Yuxi Wang, Yamei Yu, Guo-Bo Li, Shu-Ang Li, Chengyong Wu, et al.. Mechanism of microtubule stabilization by taccalonolide AJ. Nature Communications, 2017, 8, pp.15787. ⟨10.1038/ncomms15787⟩. ⟨hal-02104497⟩
123 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More