The conduit system exports locally secreted IgM from lymph nodes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Experimental Medicine Année : 2018

The conduit system exports locally secreted IgM from lymph nodes

Mirela Kuka
  • Fonction : Auteur
Giovanni de Marco
Nicolas Brouilly
Matteo Iannacone
  • Fonction : Auteur

Résumé

Immunoglobulin M (IgM) is the first type of antibody produced during acute infections and thus provides an early line of specific defense against pathogens. Being produced in secondary lymphoid organs, IgM must rapidly be exported to the blood circulation. However, it is currently unknown how such large pentameric molecules are released from lymph nodes (LNs). Here, we show that upon immunization, IgM transiently gains access to the luminal side of the conduit system, a reticular infrastructure enabling fast delivery of tissue-derived soluble substances to the LN parenchyma. Using microinjections of purified IgM, we demonstrate that conduit-associated IgM is delivered by neither the afferent lymph nor the blood, but is locally conveyed by conduits. Exploiting in vivo models, we further demonstrate that conduit-associated IgM is locally and transiently produced by activated, antigen-specific B cells migrating in the T cell zone. Thus, our study reveals that the conduit system is coopted by B cells to rapidly export secreted IgM out of LNs.

Domaines

Immunologie

Dates et versions

hal-02089004 , version 1 (03-04-2019)

Identifiants

Citer

Guilhem R. Thierry, Mirela Kuka, Giovanni de Marco, Isabelle Mondor, Nicolas Brouilly, et al.. The conduit system exports locally secreted IgM from lymph nodes. Journal of Experimental Medicine, 2018, 215 (12), pp.2972-2983. ⟨10.1084/jem.20180344⟩. ⟨hal-02089004⟩

Collections

CNRS UNIV-AMU
32 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More