Chronic abdominal vagus stimulation increased brain metabolic connectivity, reduced striatal dopamine transporter and increased mid‑brain serotonin transporter in obese miniature pigs - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Translational Medicine Année : 2019

Chronic abdominal vagus stimulation increased brain metabolic connectivity, reduced striatal dopamine transporter and increased mid‑brain serotonin transporter in obese miniature pigs

Charles-Henri Malbert
Mickael Genissel
  • Fonction : Auteur
  • PersonId : 1205260
J. L. Divoux
  • Fonction : Auteur
C. Henry
  • Fonction : Auteur

Résumé

Background/objective: Changes in brain metabolism has been investigated thoroughly during unilateral cervical chronic vagal stimulation in epileptic or depressive patients. Bilateral stimulation of the abdominal vagus (aVNS) has received less attention despite the reduction in body weight and an altered feeding behavior in obese animals that could be clinically relevant in obese individuals. Our study aims to examine the changes in brain glucose metabolism (CMRglu) induced by aVNS in obese adult miniature pigs. Dopamine (DAT) and serotonin transporters (SERT) were also quantified to further understand the molecular origins of the alterations in brain metabolism.Subjects/methods: Pairs of stimulating electrodes were implanted during laparoscopy on both abdominal vagal trunks in 20 obese adult's miniature pigs. Half of the animals were permanently stimulated while the remaining were sham stimulated. Two months after the onset of stimulation, dynamic (18)FDG PET and I-123-ioflupane SPECT were performed. Food intake, resting energy expenditure and fat deposition were also assessed longitudinally.Results: Food intake was halved and resting energy expenditure was increased by 60% in aVNS group compared to sham. The gain in body weight was also 38% less in aVNS group compared to sham. Brain metabolic connectivity increased between numerous structures including striatum, mid-brain, amygdala and hippocampus. On the contrary, increased CMRglu were restricted to the thalamus, the periaqueducal grey and the amygdala. DAT binding potential was decreased by about one third in the striatum while SERT was about doubled in the midbrain.Conclusions: Our findings demonstrated that aVNS reduced weight gain as a consequence of diminished daily food intake and increased resting energy expenditure. These changes were associated with enhanced connectivity between several brain areas. A lower striatal DAT together with a doubled mid-brain SERT were likely causative for these changes.
Fichier principal
Vignette du fichier
Malbert_2019.pdf (1.85 Mo) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...

Dates et versions

hal-02081882 , version 1 (27-03-2019)

Licence

Paternité

Identifiants

Citer

Charles-Henri Malbert, Mickael Genissel, J. L. Divoux, C. Henry. Chronic abdominal vagus stimulation increased brain metabolic connectivity, reduced striatal dopamine transporter and increased mid‑brain serotonin transporter in obese miniature pigs. Journal of Translational Medicine, 2019, 17, ⟨10.1186/s12967-019-1831-5⟩. ⟨hal-02081882⟩

Collections

INRA INRAE ALIMH
25 Consultations
36 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More