Parallel-stranded DNA with Hoogsteen base pairing stabilized by a trans-[Pt(NH3)2]2+ cross-link: characterization and conversion into a homodimer and a triplex - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biological Inorganic Chemistry Année : 2000

Parallel-stranded DNA with Hoogsteen base pairing stabilized by a trans-[Pt(NH3)2]2+ cross-link: characterization and conversion into a homodimer and a triplex

Jens Müller
  • Fonction : Auteur
Markus Drumm
  • Fonction : Auteur
Marc Boudvillain
Marc Leng
  • Fonction : Auteur
Einar Sletten
  • Fonction : Auteur
Bernhard Lippert
  • Fonction : Auteur

Résumé

The oligonucleotides 5'-d(TTTTCTTTTG) and 5'-d(AAAAGAAAAG) were cross-linked with a trans-[Pt(NH3)(2)](2+) entity via the N7 positions of the 3'-end guanine bases to give parallel-stranded (ps) DNA. At pH 4.2, CD and NMR spectroscopy indicate the presence of Hoogsteen base pairing. In addition, temperature-dependent UV spectroscopy shows an increase in melting temperature for the platinated duplex (35 degreesC) as compared to the non-platinated, antiparallel-stranded duplex formed from the same oligonucleotides (21 degreesC). A monomer-dimer equilibrium for the platinated 20mer is revealed by gel electrophoresis. At pH 4.2, addition of a third strand of composition 5'-d(AGCTTTTCTTTTAG) to the ps duplex leads to the formation of a triple helix with two distinct melting points at 38 degreesC (platinum crosslinked Hoogsteen part) and 21 degreesC (Watson-Crick part), respectively.

Dates et versions

hal-02075297 , version 1 (21-03-2019)

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Citer

Jens Müller, Markus Drumm, Marc Boudvillain, Marc Leng, Einar Sletten, et al.. Parallel-stranded DNA with Hoogsteen base pairing stabilized by a trans-[Pt(NH3)2]2+ cross-link: characterization and conversion into a homodimer and a triplex. Journal of Biological Inorganic Chemistry, 2000, 5 (5), pp.603-611. ⟨10.1007/s007750000143⟩. ⟨hal-02075297⟩
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