Effect of stirring on growth and cellulolytic enzymes production by Trichoderma harzianum in a novel bench-scale solid-state fermentation bioreactor - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Bioresource Technology Année : 2018

Effect of stirring on growth and cellulolytic enzymes production by Trichoderma harzianum in a novel bench-scale solid-state fermentation bioreactor

Résumé

A novel bench-scale stirred bioreactor for solid-state fermentation was used to determine the effect of the stirring rate on growth and enzymes production by Trichoderma harzianum PBLA. Lab-scale static tubular bioreactors were first used to assess the effect of bioreactor diameter on heat accumulation, growth, and production of cellulases and xylanases. The increased diameters (1.8-4.2 cm) led to increases in temperature up to 36 degrees C (at a rate of 1.08 degrees C/cm), which negatively affected the growth and enzyme production. Afterward, in the bench-scale bioreactor operated at rates up to 3.0 rpm, maximum xylanases production (107 +/- 0.3 U/g dm) was attained at rates of 0.5 and 1.0 rpm, reaching a maximum of 34 +/- 0.3 degrees C. Cellulases production was reduced (up to 79%) due to stirring. Therefore, the production of xylanases by T. harzianum can be performed in this cross-flow stirred SSF bioreactor at rates up to 1.0 rpm, avoiding heat accumulation and damage on metabolic activity.
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Dates et versions

hal-02073495 , version 1 (20-03-2019)

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N. Lopez-Ramirez, T. Volke-Sepulveda, Isabelle Gaime Perraud, G. Saucedo-Castaneda, E. Favela-Torres. Effect of stirring on growth and cellulolytic enzymes production by Trichoderma harzianum in a novel bench-scale solid-state fermentation bioreactor. Bioresource Technology, 2018, 265, pp.291-298. ⟨10.1016/j.biortech.2018.06.015⟩. ⟨hal-02073495⟩
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