INFOGEST static in vitro simulation of gastrointestinal food digestion - Archive ouverte HAL Access content directly
Journal Articles Nature Protocols Year : 2019

INFOGEST static in vitro simulation of gastrointestinal food digestion

1 Teagasc Food Research Centre [Fermoy, Ireland]
2 Agroscope
3 Department of Biology and Biological Engineering, Chalmers University of Technology
4 INSA - Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal]
5 NOFIMA - Norwegian Institute of Food,Fisheries and Aquaculture Research
6 Luxembourg Institute of Health
7 UMR IATE - Ingénierie des Agro-polymères et Technologies Émergentes
8 STLO - Science et Technologie du Lait et de l'Oeuf
9 BIP - Bioénergétique et Ingénierie des Protéines
10 EEZ - Estación Experimental del Zaidín
11 UCPH FOOD - Department of Food Science [Copenhagen]
12 SQPOV - Sécurité et Qualité des Produits d'Origine Végétale
13 QIB - Quadram Institute Bioscience [Norwich, U.K.]
14 Riddet Institute
15 Department of Food Engineering, Faculty of Engineering
16 Israel Institute of Technology Technion City
17 Faculty of Chemistry
18 School of Food Science & Nutrition
19 BIA - Unité de recherche sur les Biopolymères, Interactions Assemblages
20 Chenoweth Lab, Department of Food Science
21 Triskelion
22 IBET - Instituto de Biologia Experimental e Tecnológica
23 Instituto de Tecnologia Química e Biológica
24 GMPA - Génie et Microbiologie des Procédés Alimentaires
25 Departments of Biological and Agricultural Engineering, and of Food Science and Technology
26 Department of Chemistry, Biotechnology and Food Science
27 Reacta Biotech Limited
28 Ernst Moritz Arndt
29 CIAL, CSIC-UAM, CEI UAM+CSIC - Instituto de Investigación en Ciencias de la Alimentación CIAL, CSIC-UAM, CEI UAM+CSIC
Lotti Egger
  • Function : Author
Rachel Boutrou
Alfonso Clemente
Didier Dupont
Matt Golding
  • Function : Author
Olivia Ménard
Mans Minekus
  • Function : Author
Reto Portmann
  • Function : Author

Abstract

Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.
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Dates and versions

hal-02073386 , version 1 (19-03-2019)

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André Brodkorb, Lotti Egger, Marie Alminger, Paula Alvito, Ricardo Assunção, et al.. INFOGEST static in vitro simulation of gastrointestinal food digestion. Nature Protocols, 2019, 14 (4), pp.991-1014. ⟨10.1038/s41596-018-0119-1⟩. ⟨hal-02073386⟩
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