Design of metal organic framework–enzyme based bioelectrodes as a novel and highly sensitive biosensing platform
Résumé
Nanocomposites combining the mesoporous iron(III) trimesate MIL-100(Fe) (MIL: Matériaux Institut Lavoisier) and platinum nanoparticles (Pt-NPs) have been used as immobilization matrices of glucose oxidase (GOx). Due to the physico-chemical properties of Pt-NPs (electroactivity) and MIL-100(Fe) (high specific surface area and pore volume, biocompatibility), the resulting GOx–MIL-100(Fe)–PtNP bioelectrode exhibits excellent electrocatalytic performances for glucose detection. This novel glucose biosensor presents a high sensitivity of 71 mA M−1 cm−2 under optimum conditions and a low limit of detection of 5 μM with low response time (<5 s). In contrast, substitution of iron by chromium or aluminum in MIL-100 leads to a much lower sensitivity and higher response time values, suggesting that the iron centres of MIL-100(Fe) may be involved in a synergistic effect which indeed enhances the catalytic oxidation of glucose and biosensor activity. Thus, this work extends the scope of MOF nanoparticles with engineered cores and surface to the field of highly sensitive, durable glucose biosensors.
Mots clés
Platinum
High specific surface area
Glucose oxidases (GOx)
Immobilization matrices
Biocompatibility
Biosensors
Catalytic oxidation
Chemical detection
Crystalline materials
Glucose
Glucose sensors
Iron
Nanoparticles
Organometallics
Biosensing platforms
Electrocatalytic performance
Metal organic framework
Physicochemical property
Platinum nano-particles
Glucose oxidase