Article Dans Une Revue Genome Biology and Evolution Année : 2018

Phylogenomic Analysis of β-Lactamase in Archaea and Bacteria Enables the Identification of Putative New Members

Résumé

beta-lactamases are enzymes which are commonly produced by bacteria and which degrade the beta-lactam ring of beta-lactam antibiotics, namely penicillins, cephalosporins, carbapenems, and monobactams, and inactivate these antibiotics. We performed a rational and comprehensive investigation of beta-lactamases in different biological databases. In this study, we constructed hidden Markov model profiles as well as the ancestral sequence of four classes of beta-lactamases (A, B, C, and D), which were used to identify potential beta-lactamases from environmental metagenomic (1206), human microbiome metagenomic (6417), human microbiome reference genome (1310), and NCBI's nonredundant databases (44101). Our analysis revealed the existence of putative beta-lactamases in the metagenomic databases, which appeared to be similar to the four different molecular classes (A-D). This is the first report on the large-scale phylogenetic diversity of new members of beta-lactamases, and our results revealed that metagenomic database dark-matter contains beta-lactamase-like antibiotic resistance genes.

Dates et versions

hal-02057304 , version 1 (05-03-2019)

Identifiants

Citer

Vivek Keshri, Arup Panda, Anthony Levasseur, Jean-Marc Rolain, Pierre Pontarotti, et al.. Phylogenomic Analysis of β-Lactamase in Archaea and Bacteria Enables the Identification of Putative New Members. Genome Biology and Evolution, 2018, 10 (4), pp.1106-1114. ⟨10.1093/gbe/evy028⟩. ⟨hal-02057304⟩
93 Consultations
0 Téléchargements

Altmetric

Partager

  • More