Grasslands are globally extensive; they exist in many different climates, at high and low elevations, on nutrient-rich and nutrient-poor soils. Grassland distributions today are closely linked to human activities, herbivores, and fire, but many have been converted to urban areas, forests, or agriculture fields. Roughly 80% of fires globally occur in grasslands each year, making fire a critical process in grassland dynamics. Yet, little is known about the long-term history of fire in grasslands. Here, we analyze sedimentary archives to reconstruct grassland fire histories during the Holocene. Given that grassland locations change over time, we compare several charcoal-based fire reconstructions based on alternative classification schemes: (a) sites from modern grassland locations; (b) sites that were likely grasslands during the mid-Holocene; and (c) sites based on author-derived classifications. We also compare fire histories from grassland sites, forested sites, and all sites globally over the past 12,000years. Forested versus grassland sites show different trends: grassland burning increased from the early to mid-Holocene, reaching a maximum about 8000-6000years ago, and subsequently declined, reaching a minimum around 4000years ago. In contrast, biomass burning in forests increased during the Holocene until about 2000years ago. Continental grassland fire history reconstructions show opposing Holocene trends in North versus South America, whereas grassland burning in Australia was highly variable in the early Holocene and much more stable after the mid-Holocene. The sharp differences in continental as well as forest versus grassland Holocene fire history trajectories have important implications for our understanding of global biomass burning and its emissions, the global carbon cycle, biodiversity, conservation, and land management.