Defective T-cell receptor gamma gene rearrangement in interleukin-7 receptor knockout mice. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Immunology Letters Année : 1997

Defective T-cell receptor gamma gene rearrangement in interleukin-7 receptor knockout mice.

J Peschon
  • Fonction : Auteur
K Muegge
  • Fonction : Auteur
S Durum
  • Fonction : Auteur

Résumé

T-cell receptor (TCR) genes need to be rearranged by a site specific-VDJ recombinase before they are expressed. This process, initiated in CD44+25+ thymocytes, takes place during the early stage of T-cell differentiation in the thymus. Interleukin-7 receptor alpha chain knockout (IL-7R-/-) mice are severely deficient in B-lymphocytes and alpha beta T-cells and completely lack the gamma delta T-cell lineage. Thymocyte development is arrested at a very early stage (DN CD44+CD25-). Because this arrest is earlier than in mice with a block in VDJ recombination, we examined the rearrangement status of TCR genes in thymocytes from IL-7R-/- mice. The TCR beta locus showed a nearly normal pattern of VDJ rearrangements, consistent with the presence of alpha beta T-cells in these mice. However, TCR gamma locus rearrangement was absent or severely reduced for all the V gamma genes analyzed (V gamma 3, V gamma 4, V gamma 1.1, V gamma 1.2 and V gamma 2). In contrast, the delta locus showed little reduction in rearrangement. The defect in gamma rearrangements in IL-7R-/- thymocytes is not simply due to an absence of mature gamma delta T-cells, since TCR delta-/- mice, which also have only alpha beta T-cells, had normal levels of gamma and delta rearrangements. These findings indicate that one or both of the two known ligands of IL-7R, IL-7 and thymic stromal lymphopoietin (TSLP) serves as an extrinsic signal to specifically rearrange the TCR gamma locus.
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Dates et versions

hal-01949109 , version 1 (09-12-2018)

Identifiants

  • HAL Id : hal-01949109 , version 1
  • PUBMED : 9232418

Citer

Serge M. Candéias, J Peschon, K Muegge, S Durum. Defective T-cell receptor gamma gene rearrangement in interleukin-7 receptor knockout mice.. Immunology Letters, 1997, 57 (1-3), pp.9-14. ⟨hal-01949109⟩
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