High chlorpyrifos resistance in Culex pipiens mosquitoes: strong synergy between resistance genes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Heredity Année : 2016

High chlorpyrifos resistance in Culex pipiens mosquitoes: strong synergy between resistance genes

Résumé

We investigated the genetic determinism of high chlorpyrifos resistance (HCR), a phenotype first described in 1999 in Culex pipiens mosquitoes surviving chlorpyrifos doses 1 mg l−1 and more recently found in field samples from Tunisia, Israel or Indian Ocean islands. Through chlorpyrifos selection, we selected several HCR strains that displayed over 10 000-fold resistance. All strains were homozygous for resistant alleles at two main loci: the ace-1 gene, with the resistant ace-1R allele expressing the insensitive G119S acetylcholinesterase, and a resistant allele of an unknown gene (named T) linked to the sex and ace-2 genes. We constructed a strain carrying only the T-resistant allele and studied its resistance characteristics. By crossing this strain with strains harboring different alleles at the ace-1 locus, we showed that the resistant ace-1R and the T alleles act in strong synergy, as they elicited a resistance 100 times higher than expected from a simple multiplicative effect. This effect was specific to chlorpyrifos and parathion and was not affected by synergists. We also examined how HCR was expressed in strains carrying other ace-1-resistant alleles, such as ace-1V or the duplicated ace-1D allele, currently spreading worldwide. We identified two major parameters that influenced the level of resistance: the number and the nature of the ace-1-resistant alleles and the number of T alleles. Our data fit a model that predicts that the T allele acts by decreasing chlorpyrifos concentration in the compartment targeted in insects.
Fichier principal
Vignette du fichier
alout_heredity_2016_1.pdf (653.86 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01944470 , version 1 (27-05-2020)

Identifiants

Citer

Haoues Alout, Pierrick Labbé, A Berthomieu, Patrick Makoundou, Philippe Fort, et al.. High chlorpyrifos resistance in Culex pipiens mosquitoes: strong synergy between resistance genes. Heredity, 2016, 116 (2), pp.224-231. ⟨10.1038/hdy.2015.92⟩. ⟨hal-01944470⟩
167 Consultations
62 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More