Expression of glutamine synthetase genes in roots and nodules of Phaseolus vulgaris following changes in the ammonium supply and infection with various Rhizobium mutants - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant Molecular Biology Reporter Année : 1990

Expression of glutamine synthetase genes in roots and nodules of Phaseolus vulgaris following changes in the ammonium supply and infection with various Rhizobium mutants

J. Mark Cock
Ruth M Mould
  • Fonction : Auteur
Julie V Cullimore

Résumé

In this paper we have examined whether the four glutamine synthetase (gin) genes, expressed in roots and nodules ofPhaseolus vulgaris are substrate-inducible by ammonium. Manipulation of the ammonium pool in roots, through addition and removal of exogenous ammonium, did not elicit any changes in the abundances of the four mRNAs thus suggesting that the gin genes in roots of this legume are neither substrate-inducible by ammonium nor derepressed during nitrogen starvation. In nodules the effect of the ammonium supply on expression of the gin genes has been examined by growing nodules under argon/oxygen atmospheres, or with a number of Fix-Rhizobium mutants, and following addition of exogenous ammonium. The results of these experiments suggest that the expression of the gln-7 gene, which is strongly induced during nodule development, is primarily under a developmental control. However nitrogen fixation appears to have a quantitative effect on expression ofgln-7 as the abundance of this mRNA is about 2 to 4-fold higher under nitrogen-fixing conditions. This effect could not be mimicked by addition of exogenous ammonium and moreover is not specific to the gln-y gene as mRNA from a leghaemoglobin gene was similarly affected. Taken together these results have failed to fred an effect of ammonium on specifically inducing the expression of glutamine synthetase genes in roots and nodules of P. vulgaris.
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Dates et versions

hal-01926706 , version 1 (19-11-2018)

Identifiants

  • HAL Id : hal-01926706 , version 1

Citer

J. Mark Cock, Ruth M Mould, Malcolm Bennett, Julie V Cullimore. Expression of glutamine synthetase genes in roots and nodules of Phaseolus vulgaris following changes in the ammonium supply and infection with various Rhizobium mutants. Plant Molecular Biology Reporter, 1990, 14, pp.549 - 560. ⟨hal-01926706⟩
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