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Article Dans Une Revue Plant Molecular Biology Reporter Année : 1991

Regulation of glutamine synthetase genes in leaves of Phaseolus vulgaris

J. Mark Cock
Ian W Brock
  • Fonction : Auteur
Adam T Watson
  • Fonction : Auteur
Andrew P Morby
  • Fonction : Auteur
Julie V Cullimore

Résumé

Glutamine synthetase (GS) activity increased over threefold in developing primary leaves of Phaseolus vulgaris L. This increase was shown to be the result of differential expression of three members of the GS gene family: gln-c~ and gln-[3, which encode cytosolic GS polypeptides, and gln-b, which encodes the chloroplast-located GS. The gln-b gene was the most highly expressed GS gene and was regulated in a complex manner with two different transcripts accumulating differentially during leaf development. This gene was expressed weakly in the dark and was induced strongly by light; this induction was shown not to be an indirect effect of photorespiration. In the long term, gln-b showed increased expression in photorespiring compared with non-photorespiring leaves. However, in the short term, there was no induction of gln-b following transfer of plants to photorespiratory conditions. These results suggest that regulation of gln-b by photorespiration was the result of indirect, long-term effects on cellular metabolism. In general, in all these experiments, analysis of cytosolic versus chloroplastic GS polypeptides and of the GS isoenzyme profiles showed the same pattern of changes in abundance as that observed for the mRNAs suggesting that regulation of GS gene expression occurred primarily at the mRNA level. However, it was noteworthy that the b isoenzyme remained at a high abundance in older leaves, grown in both light and dark, despite a decrease in abundance of gln-b mRNA.
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Dates et versions

hal-01926699 , version 1 (19-11-2018)

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  • HAL Id : hal-01926699 , version 1

Citer

J. Mark Cock, Ian W Brock, Adam T Watson, Ranjan Swarup, Andrew P Morby, et al.. Regulation of glutamine synthetase genes in leaves of Phaseolus vulgaris. Plant Molecular Biology Reporter, 1991, 17, pp.761 - 771. ⟨hal-01926699⟩
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