Characterization of the gene encoding the plastid-located glutamine synthetase of Phaseolus vulgaris: regulation of/-glucuronidase gene fusions in transgenic tobacco - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant Molecular Biology Reporter Année : 1992

Characterization of the gene encoding the plastid-located glutamine synthetase of Phaseolus vulgaris: regulation of/-glucuronidase gene fusions in transgenic tobacco

J. Mark Cock
Pascale Hémon
  • Fonction : Auteur
Julie V Cullimore

Résumé

The gln-~ gene, which encodes the plastid-located glutamine synthetase ofPhaseolus vulgaris, was cloned and its promoter region was sequenced. Primer extension analysis was used to map the two major transcription initiation sites which are about 90 nucleotides apart. A fusion of 2.3 kb of the upstream region of the gln-b gene to the reporter gene uidA encoding/?-glucuronidase was shown to be expressed in the chlorophyllous cell types of leaves and stems and in the root meristem region of transgenic tobacco. Analysis of a series of three 5' promoter deletion fusions revealed the presence of a region essential for promoter activity between-786 and-327 and regions involved in tissue-specific regulation and light regulation between-786 and + 43.
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Dates et versions

hal-01926686 , version 1 (19-11-2018)

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  • HAL Id : hal-01926686 , version 1

Citer

J. Mark Cock, Pascale Hémon, Julie V Cullimore. Characterization of the gene encoding the plastid-located glutamine synthetase of Phaseolus vulgaris: regulation of/-glucuronidase gene fusions in transgenic tobacco. Plant Molecular Biology Reporter, 1992, 18, pp.1141 - 1149. ⟨hal-01926686⟩
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