Natural antisense transcripts of the S locus receptor kinase gene and related sequences in Brassica oleracea - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular and General Genetics MGG Année : 1997

Natural antisense transcripts of the S locus receptor kinase gene and related sequences in Brassica oleracea

Résumé

Gene expression can be inhibited by antisense RNA transcripts. Although this phenomenon is widely used to analyse gene function in plants, the molecular mechanisms involved are poorly understood. One approach to improving our understanding of antisense gene regulation is to analyse the function of endogenous antisense transcripts. To date, only a small number of plant genes have been shown to be transcribed in both directions and limited information is available concerning the role of natural antisense transcripts in plants. In this study, we have identi®ed several natural antisense transcripts which hybridise to probes derived from the S locus receptor kinase gene (SRK). The RNase protection assay and reverse trancriptase-PCR were used to demonstrate that a proportion of the antisense transcripts are encoded directly by SRK. Using di€erent RNase protection probes, regions of the promoter, exon I (which encodes the S domain) and intron I of SRK were shown to be transcribed in an antisense direction. An antisense SRK transcript was shown to inhibit translation of a sense transcript in vitro. The possible role of antisense SRK transcripts in vivo is discussed.

Dates et versions

hal-01926580 , version 1 (19-11-2018)

Identifiants

Citer

J. Mark Cock, Swarup Ranjan, Dumas Christian. Natural antisense transcripts of the S locus receptor kinase gene and related sequences in Brassica oleracea. Molecular and General Genetics MGG, 1997, 255 (5), pp.514-524. ⟨10.1007/s004380050524⟩. ⟨hal-01926580⟩
8 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More