Early hypothalamic activation of combined fos and CRH41 immunoreactivity and of CRH41 release in push-pull cannulated rats after systemic endotoxin challenge - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular and Chemical Neuropathology Année : 1995

Early hypothalamic activation of combined fos and CRH41 immunoreactivity and of CRH41 release in push-pull cannulated rats after systemic endotoxin challenge

G Givalois
  • Fonction : Auteur
P. Siaud
  • Fonction : Auteur
M. Mekaouche
  • Fonction : Auteur
G. Ixart
  • Fonction : Auteur
F. Malaval
  • Fonction : Auteur
I. Assenmacher
  • Fonction : Auteur

Résumé

We previously showed that intra-arterial endotoxin infusion (lipopolysaccharide [LPS]: 25 micrograms.kg-1) induced an early (15 min) and sustained (480 min) rise in plasma ACTH associated with delayed (60-120 min) increases in plasma concentrations of TNF alpha, IL-6, and IL-1 beta. In the present study, we followed the post-LPS time-course of immunocytochemical expression of Fos-like activity in CRH41 neurons whose immunolabeling was enhanced by icv colchicine pretreatment 48 h before the LPS, and CRH41 release in the push-pull cannulated median eminence of free-moving rats, in parallel with the ACTH response. The earliest Fos-like activity in IR-CHR41 neurons was detected 30 min post-LPS. Colchicine strongly inhibited the LPS-induced activation of Fos expression in single-labeled paraventricular neurons. CRH41 release in the median eminence displayed a biphasic stimulation pattern, with a first peak (+60%) at 15 min together with the ACTH surge, followed by a second rise beginning at 45 min and lasting more than 2 h. Thus, the early stage of the ACTH surge following a nonlethal endotoxin challenge (< 60 min) already involves the activation of CRH41-producing neurons.

Dates et versions

hal-01923639 , version 1 (15-11-2018)

Identifiants

Citer

G Givalois, P. Siaud, M. Mekaouche, G. Ixart, F. Malaval, et al.. Early hypothalamic activation of combined fos and CRH41 immunoreactivity and of CRH41 release in push-pull cannulated rats after systemic endotoxin challenge. Molecular and Chemical Neuropathology, 1995, 26 (2), pp.171 - 186. ⟨10.1007/BF02815011⟩. ⟨hal-01923639⟩
63 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More