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Article Dans Une Revue Optics Express Année : 2018

Wide field fluorescence epi-microscopy behind a scattering medium enabled by speckle correlations

Matthias Hofer
Sophie Brasselet

Résumé

Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and exploit the optical memory effect to enable fluorescence microscopy through a turbid layer. We demonstrate efficient reconstruction of micrometer-size fluorescent objects behind a scattering medium in epi-microscopy, and study the specificities of this imaging modality (magnification, field of view, resolution) as compared to traditional microscopy. Using a modified phase retrieval algorithm to reconstruct fluorescent objects from speckle images, we demonstrate robust reconstructions even in relatively low signal to noise conditions. This modality is particularly appropriate for imaging in biological media, which are known to exhibit relatively large optical memory ranges compatible with tens of micrometers size field of views, and large spectral bandwidths compatible with emission fluorescence spectra of tens of nanometers widths. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
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Dates et versions

hal-01912524 , version 1 (05-11-2018)

Identifiants

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Matthias Hofer, Christian Soeller, Sophie Brasselet, Jacopo Bertolotti. Wide field fluorescence epi-microscopy behind a scattering medium enabled by speckle correlations. Optics Express, 2018, 26 (8), pp.9866. ⟨10.1364/OE.26.009866⟩. ⟨hal-01912524⟩
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