Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus Retina. - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2018

Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus Retina.

Résumé

Xenopus is an attractive model system for regeneration studies, as it exhibits an extraordinary regenerative capacity compared to mammals. It is commonly used to study body part regeneration following amputation, for instance of the limb, the tail, or the retina. Models with more subtle injuries are also needed for human degenerative disease modeling, allowing for the study of stem cell recruitment for the regeneration of a given cellular subtype. We present here a model to ablate photoreceptor cells in the Xenopus retina. This method is based on the nitroreductase/metronidazole (NTR/MTZ) system, a combination of chemical and genetic tools, allowing for the conditional ablation of targeted cells. This type of approach establishes Xenopus as a powerful model to study cellular regeneration and stem cell regulation.
Fichier non déposé

Dates et versions

hal-01884513 , version 1 (01-10-2018)

Identifiants

Citer

Albert Chesneau, Odile Bronchain, Muriel Perron. Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus Retina.. Kris Vleminckx. Xenopus, 1865, Springer/Humana Press, pp.133-146, 2018, Methods in Molecular Biology, 978-1-4939-8783-2. ⟨10.1007/978-1-4939-8784-9_10⟩. ⟨hal-01884513⟩
31 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More