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Article Dans Une Revue Process Biochemistry Année : 2018

Molecular rules for selectivity in lipase-catalysed acylation of lysine

Résumé

The selectivity of L-lysine acylation by lauric acid catalysed by Candida antarctica lipase B (CALB) was investigated combining experimental and theoretical methodologies. Experiments showed the near-exclusive acylation of lysine ε-amino group; only traces of product resulting from the acylation of lysine α-amino group were observed fleetingly. Molecular modelling simulations were performed aiming to understand the molecular rules for selectivity. Flexible docking simulations combined with structural investigations into lysine/CALB binding modes also suggested the preferential acylation of lysine ε-amino group without, however, excluding the acylation of the lysine α-amino group. Electrostatic interaction energy between lysine and the residues covering the catalytic cavity was calculated in order to understand the discrimination between the two lysine amino groups. The results suggests that the proximity of the carboxylate group hinders the binding of the substrate in configurations enabling the N-acylation. Key interactions with the polar region covering the catalytic triad were identified and a plausible explanation for selectivity was proposed.
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Dates et versions

hal-01883545 , version 1 (19-08-2019)

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Léna Dettori, Christian Jelsch, Yann Guiavarc’h, Stéphane Delaunay, Xavier Framboisier, et al.. Molecular rules for selectivity in lipase-catalysed acylation of lysine. Process Biochemistry, 2018, 74, pp.50 - 60. ⟨10.1016/j.procbio.2018.07.021⟩. ⟨hal-01883545⟩
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