Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients - Archive ouverte HAL
Article Dans Une Revue Journal of Clinical Microbiology Année : 2016

Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

Résumé

The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/10(6) peripheral blood mononuclear cells.

Dates et versions

hal-01872543 , version 1 (12-09-2018)

Identifiants

Citer

Sylvie Zida, Edouard Tuaillon, Makoura Barro, Arnaud Kwimatouo Lekpa Franchard, Thérèse Kagoné, et al.. Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients. Journal of Clinical Microbiology, 2016, 54 (6), pp.1641 - 1643. ⟨10.1128/JCM.03372-15⟩. ⟨hal-01872543⟩
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