Journal Articles Methods in Molecular Biology Year : 2018

High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.

Abstract

Chromatin immunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is widely used for studying the nucleoprotein components that are involved in the various cellular processes required for shaping the bacterial nucleoid. This methodology, termed ChIP-sequencing (ChIP-seq), enables the identification of the DNA targets of DNA binding proteins across genome-wide maps. Here, we describe the steps necessary to obtain short, specific, high-quality immunoprecipitated DNA prior to DNA library construction for NGS and high-resolution ChIP-seq data.

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hal-01849465 , version 1 (05-06-2019)

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Roxanne Diaz, Aurore Sanchez, Véronique Le Berre, Jean-Yves Bouet. High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.. Methods in Molecular Biology, 2018, pp.61-73. ⟨10.1007/978-1-4939-7098-8_6⟩. ⟨hal-01849465⟩
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