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Article Dans Une Revue Bioanalysis Année : 2017

Quantification of hepcidin-25 in human cerebrospinal fluid using LC–MS/MS

Résumé

AIM: Hepcidin, the main iron metabolism regulator, can be detected in various biological fluids. Here, we describe a quantitative method of LC-MS/MS to quantify the 25 amino acid isoform of hepcidin (hepcidin-25) in human cerebrospinal fluid (CSF). Results & methodology: Samples were prepared through protein precipitation followed by solid phase extraction (SPE) and injected into a triple-quadrupole mass spectrometer. Validation of our method included determination of LOQ (0.1 ng/ml), repeatability, intermediate precision, recovery and linearity (up to 25 ng/ml). Hepcidin-25 was subsequently quantified in 36 human CSF samples and its concentration ranged from 0.21 to 3.54 ng/ml. CONCLUSION: This is the first time that hepcidin-25 can be reliably quantified in human CSF. This may open interesting perspectives for the management of iron-related neurological disorders.
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Dates et versions

hal-01792350 , version 1 (15-05-2018)

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Constance Delaby, Pauline Bros, Jérôme Vialaret, Amandine Moulinier, Vincent Delatour, et al.. Quantification of hepcidin-25 in human cerebrospinal fluid using LC–MS/MS. Bioanalysis, 2017, 9 (4), pp.337 - 347. ⟨10.4155/bio-2016-0240⟩. ⟨hal-01792350⟩
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