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Article Dans Une Revue Environmental Science and Technology Année : 2005

Metabolism of [14C]-2,4,6-trinitrotoluene in tobacco cell suspension cultures

Mireille Vila
  • Fonction : Auteur
Sophie Lorber
Estelle Rathahao-Paris
Laurent Debrauwer
Cécile Canlet
Francois Laurent

Résumé

The metabolism of 2,4,6-trinitrotoluene (TNT) was investigated in tobacco cell suspension cultures amended with [14C]-TNT. Five metabolites were purified and characterized. Temporal evolution of metabolites was monitored during a 120 h incubation period. Metabolites structure was identified by acid and enzymatic hydrolysis, followed by electrospray ionization mass spectrometry and 1H and 13C NMR spectroscopy analyses. The majority of metabolites were conjugates formed by glycose conjugation on the hydroxylamine group of either 2-hydroxylamino-4,6-dinitrotoluene (2-HADNT) or 4-hydroxylamino-2,6-dinitrotoluene (4-HADNT), which led to monoglycoside then to diglycoside. Various diglycosides were observed with gentiobioside or sophoroside formation. Bound residues represented a small fraction (<10% of initial 14C) irrespective of the interval after TNT amendment. Free ADNT was detected only in the medium. This study highlights the central role played by HADNT in the TNT metabolic pathway in tobacco cell suspension culture, and the key role of these compounds and of glycosyltransferases in TNT phytoremediation processes.
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Dates et versions

hal-01763222 , version 1 (10-04-2018)

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Mireille Vila, Sophie Lorber, Estelle Rathahao-Paris, Laurent Debrauwer, Cécile Canlet, et al.. Metabolism of [14C]-2,4,6-trinitrotoluene in tobacco cell suspension cultures. Environmental Science and Technology, 2005, 39 (2), pp.663-72. ⟨hal-01763222⟩
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