Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Infection and Immunity Année : 2017

Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis

Résumé

Mycobacterium canettii, which has a smooth colony morphology, is the tuberculous organism retaining the most genetic traits from the putative last common ancestor of the rough-morphology Mycobacterium tuberculosis complex. To explore whether M. canettii can infect individuals by the oral route, mice were fed phosphate-buffered saline or 106 M. canettii mycobacteria and sacrificed over a 28-day experiment. While no M. canettii was detected in negative controls, M. canettii-infected mice yielded granuloma-like lesions for 4/4 lungs at days 14 and 28 postinoculation (p.i.) and positive PCR detection of M. canettii for 5/8 mesenteric lymph nodes at days 1 and 3 p.i. and 5/6 pooled stools collected from day 1 to day 28 p.i. Smooth M. canettii colonies grew from 68% of lungs and 36% of spleens and cervical lymph nodes but fewer than 20% of axillary lymph nodes, livers, brown fat samples, kidneys, or blood samples throughout the 28-day experiment. Ready translocation in mice after digestive tract challenge demonstrates the potential of ingested M. canettii organisms to relocate to distant organs and lungs. The demonstration of this relocation supports the possibility that populations may be infected by environmental M. canettii.

Dates et versions

hal-01729789 , version 1 (12-03-2018)

Identifiants

Citer

Feriel Bouzid, Fabienne Bregeon, Hubert Lepidi, Helen D. Donoghue, David E. Minnikin, et al.. Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis. Infection and Immunity, 2017, 85 (12), ⟨10.1128/IAI.00507-17⟩. ⟨hal-01729789⟩
26 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More