Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences - Archive ouverte HAL Access content directly
Journal Articles Chemical Science Year : 2017

Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences

Abstract

We report the design of a luminescent sensor based upon the zinc finger (ZF) protein TIS11d, that allows for the selective time-resolved detection of the UUAUUUAUU sequence of the 3 0-untranslated region of messenger RNA. This sensor is composed of the tandem ZF RNA binding domain of TIS11d functionalized with a luminescent Tb 3+ complex on one of the ZFs and a sensitizing antenna on the other. This work provides the proof of principle that an RNA binding protein can be re-engineered as an RNA sensor and, more generally, that tunable synthetic luminescent probes for biomolecules can be obtained by modifying biomolecule-binding domains.
Fichier principal
Vignette du fichier
seneque chem Sciences.pdf (781.65 Ko) Télécharger le fichier
Origin Publisher files allowed on an open archive
Loading...

Dates and versions

hal-01692935 , version 1 (25-01-2018)

Identifiers

Cite

Laurent Raibaut, William Vasseur, Geoffrey D Shimberg, Christine Saint-Pierre, Jean-Luc Ravanat, et al.. Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences. Chemical Science, 2017, 8 (2), pp.1658 - 1664. ⟨10.1039/c6sc04086a⟩. ⟨hal-01692935⟩
151 View
99 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More