Physicochemical regulation of TGF and VEGF delivery from mesoporous calcium phosphate bone substitutes
Résumé
Determination of the physicochemical parameters governing growth factors
(GFs) adsorption and release from mesoporous calcium phosphate ceramics. Materials
& methods: Six mesoporous calcium phosphate ceramics prepared by soft and hard
templating were loaded with two different physiological concentrations of TGF-β1 or
VEGF165 and their in vitro kinetics of adsorption/release were studied. Results: This low
GF loading promotes adsorption on the highest binding sites. The usually encountered
detrimental burst release is thus considerably reduced for samples prepared by hardtemplating
method. Conclusion: Our findings highlight that the strong affinity of GFs
with the ceramic surfaces, demonstrated by a slow GFs release, is enhanced by the
large surface area, confinement into mesopores of ceramics and high difference of
surface charge between ceramic surfaces and GFs