Genome sequencing and mapping reveal loss of heterozygosity as a mechanism for rapid adaptation in the vegetable pathogen Phytophthora capsici. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular Plant-Microbe Interactions Année : 2012

Genome sequencing and mapping reveal loss of heterozygosity as a mechanism for rapid adaptation in the vegetable pathogen Phytophthora capsici.

Sylvain Raffaele
Ryan S Donahoo
  • Fonction : Auteur
Joe Win
Sophien Kamoun

Résumé

The oomycete vegetable pathogen Phytophthora capsici has shown remarkable adaptation to fungicides and new hosts. Like other members of this destructive genus, P. capsici has an explosive epidemiology, rapidly producing massive numbers of asexual spores on infected hosts. In addition, P. capsici can remain dormant for years as sexually recombined oospores, making it difficult to produce crops at infested sites, and allowing outcrossing populations to maintain significant genetic variation. Genome sequencing, development of a high-density genetic map, and integrative genomic or genetic characterization of P. capsici field isolates and intercross progeny revealed significant mitotic loss of heterozygosity (LOH) in diverse isolates. LOH was detected in clonally propagated field isolates and sexual progeny, cumulatively affecting >30% of the genome. LOH altered genotypes for more than 11,000 single-nucleotide variant sites and showed a strong association with changes in mating type and pathogenicity. Overall, it appears that LOH may provide a rapid mechanism for fixing alleles and may be an important component of adaptability for P. capsici.

Dates et versions

hal-01608652 , version 1 (03-10-2017)

Identifiants

Citer

Kurt H Lamour, Joann Mudge, Daniel Gobena, Oscar P Hurtado-Gonzales, Jeremy Schmutz, et al.. Genome sequencing and mapping reveal loss of heterozygosity as a mechanism for rapid adaptation in the vegetable pathogen Phytophthora capsici.. Molecular Plant-Microbe Interactions, 2012, 25 (10), pp.1350-60. ⟨10.1094/MPMI-02-12-0028-R⟩. ⟨hal-01608652⟩
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