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Poster De Conférence Année : 2017

Inoculation of Wheat dwarf virus strains to wheat and barley plants by Psammotettix alienus

Résumé

Wheat dwarf virus (WDV) infects plants of the Poaceae family. WDV species includes the WDV-wand the WDV-b strains. Contradictory data present in the literature led to a conclusion that WDV wand WDV-b preferentially infect wheat and barley, respectively. WDV is transmitted by the leaf hopper Psammotettix alienus. However, species identification in the genus Psammotettix, based on morphometric characterization of the aedeagus, is a challenging procedure. Indeed, due to high aedeagus variability, species identification can lead to the incorrect assignment of males.More over, it makes impossible the identification of larvae and females. Thus, in some published works on WDV, it is possible that leafhoppers were not accurately characterized before use in transmission experiments. To improve knowledge on WDV host range, our aim was to describe Psammotettix leafhoppers at the species level using multiple criteria and then to use P. alienus to inoculate WDV to wheat and barley. Leafhopper characterization included recordings of vibrational signals used during mating and morphometric analyses. In addition, sequences of the COI gene were obtained and compared to sequences available in databases. Field-collected P.alienus were characterized, reared and then used in WDV transmission experiments. Larvae, males and females were tested for their ability to transmit WDV strains. Moreover, the abilities of WDV strains to infect wheat and barley plants was tested using host-alternation procedures based on single and dual viral acquisitions/inoculations. Inoculation of WDV-w and WDV-b to barley and wheat, respectively, failed to produce infected plants while WDV-w/WDV-b dual inoculation made it possible to detect WDV-w in barley and WDV-b in wheat. These results suggest a helper effect between WDV strains during host infection. Such information is crucial for future work on the epidemiology of wheat dwarf disease.
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Dates et versions

hal-01606614 , version 1 (02-10-2017)

Identifiants

  • HAL Id : hal-01606614 , version 1
  • PRODINRA : 396921

Citer

Isabelle Abt, Marlène Souquet, Maja Derlink, Annie de Keyzer, Marc Letroublon, et al.. Inoculation of Wheat dwarf virus strains to wheat and barley plants by Psammotettix alienus. 3rd Hemipteran-Plant Interactions Symposium (HPIS 2017), Jun 2017, Madrid, Spain. 2017, HPIS 2017 3rd Hemipteran-Plant Interactions Symposium: book of abstracts. ⟨hal-01606614⟩
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