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Poster De Conférence Année : 2017

Characterization of the host range of barley and wheat strains of Wheat dwarf virus

Résumé

Wheat dwarf virus (WDV, Geminiviridae family, Mastrevirus genus) is the causal agent of dwarfing, mottling and yellowing symptoms in wheat and barley. WDV is exclusively transmitted by leafhoppers from the genus Psammotettix in a persistent manner. The circular single-stranded DNA genome of WDV, of about 2.75-kb long, encodes two replication-associated proteins, the movement protein and the coat protein. The WDV genome also contains two non-coding sequences that are important for viral replication and regulation of gene expression. Phylogenetic analyses carried out with WDV sequences showed that this viral species comprises several strains including the originally described wheat (WDV) and barley (WDV-Bar) strains. The WDV strains share 83-84% nucleotide identity. WDV isolates infect plants of the Poaceae family; however i) the host ranges of the WDV strains are not completely resolved, ii) contradictory results associated to virus-host interactions are present in databases, iii) WDV and WDV-Bar isolates have wheat and barley as their preferred host, respectively, and iv) each strain can infect, at least occasionally, the preferred host of the other strain. In this study, host ranges of isolates from the WDV and WDV-Bar strains were determined using well characterized isolates and Psammotettix alienus leafhoppers. Thirty-seven wild gramineous species have been reported in a 5-km² site located in Annoix (Cher, France) (Gauthier et al., 2017). Whenever seeds were available, susceptibility of these wild gramineous species to WDV was tested using leafhopper-mediated inoculations. The results show that the two WDV strains have different but slightly overlapping host ranges. Moreover, our results revealed variations for some parameters of viral infection (e.g. susceptibility and latent period in infected plant) according to host species/WDV strain combinations. To further characterize the viral molecular determinant(s) involved in WDV host range, WDV and WDV-Bar infectious clones were constructed. These tools will enable, through the production of artificial mutants/recombinants, to determine the genetic determinants of the specific biological properties of each WDV strain, including the ability to infect a given host species.

Domaines

Virologie
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Dates et versions

hal-01606281 , version 1 (02-10-2017)

Identifiants

  • HAL Id : hal-01606281 , version 1
  • PRODINRA : 404118

Citer

Isabelle Abt, Marlène Souquet, Maja Derlink, Coline Temple, Kevin Gauthier, et al.. Characterization of the host range of barley and wheat strains of Wheat dwarf virus. 16è Rencontres de Virologie Végétale (RVV 2017), Jan 2017, Aussois, France. , 2017. ⟨hal-01606281⟩
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