Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 2007

Human cellular prion protein hPrPC is sorted to the apical membrane of epithelial cells

Résumé

Propagation of the scrapie isoform of the prion protein (PrP(Sc)) depends on the expression of endogenous cellular prion (PrP(C)). During oral infection, PrP(Sc) propagates, by conversion of the PrP(C) to PrP(Sc), from the gastrointestinal tract to the nervous system. Intestinal epithelium could serve as the primary site for PrP(C) conversion. To investigate PrP(C) sorting in epithelia cells, we have generated both a green fluorescent protein (EGFP) or hemagglutinin (HA) tagged human PrP(C) (hPrP(C)). Combined molecular, biochemical, and single living polarized cell imaging characterizations suggest that hPrP(C) is selectively targeted to the apical side of Madin-Darby canine kidney (MDCKII) and of intestinal epithelia (Caco2) cells.

Dates et versions

hal-01606069 , version 1 (02-10-2017)

Identifiants

Citer

B. de Keukeleire, S. Donadio, J. Micoud, Delphine Lechardeur, M. Benharouga. Human cellular prion protein hPrPC is sorted to the apical membrane of epithelial cells. Biochemical and Biophysical Research Communications, 2007, 354 (4), pp.949-954. ⟨10.1016/j.bbrc.2007.01.096⟩. ⟨hal-01606069⟩
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