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Article Dans Une Revue Plant Physiology Année : 2017

Rice sucrose partitioning mediated by a putative pectin methyltransferase and homogalacturonan methylesterification

Résumé

Homogalacturonan (HG) is the main component of pectins. HG methylesterification has recently emerged as a key determinant controlling cell attachment, organ formation, and phyllotaxy. However, whether and how HG methylesterification affects intercellular metabolite transport has rarely been reported. Here, we identified and characterized knockout mutants of the rice (Oryza sativa) OsQUA2 gene encoding a putative pectin methyltransferase. Osqua2 mutants exhibit a remarkable decrease in the degree of methylesterification of HG in the culm-sieve element cell wall and a markedly reduced grain yield. The culm of Osqua2 mutant plants contains excessive sucrose (Suc), and a 13CO2 feeding experiment showed that the Suc overaccumulation in the culm was caused by blocked Suc translocation. These and other findings demonstrate that OsQUA2 is essential for maintaining a high degree of methylesterification of HG in the rice culm-sieve element cell wall, which may be critical for efficient Suc partitioning and grain filling. In addition, our results suggest that the apoplastic pathway is involved in longdistance Suc transport in rice. The identification and characterization of the OsQUA2 gene and its functionality revealed a previously unknown contribution of HG methylesterification and provided insight into how modification of the cell wall regulates intercellular transport in plants.

Dates et versions

hal-01604454 , version 1 (02-10-2017)

Identifiants

Citer

Yonghan Xu, Julien Sechet, Yingbao Wu, Yaping Fu, Longfei Zhu, et al.. Rice sucrose partitioning mediated by a putative pectin methyltransferase and homogalacturonan methylesterification. Plant Physiology, 2017, 174 (3), pp.1595-1608. ⟨10.1104/pp.16.01555⟩. ⟨hal-01604454⟩
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