MICROSCOPY | Confocal Laser Scanning Microscopy
Résumé
Confocal laser scanning microscopy (CLSM) is a versatile fluorescence-imaging tool that can visualize in situ microorganisms in their three-dimensional environments and probe their natural microhabitats (biofilms, food matrix, gut, etc.). It enables the analysis of the dynamics of microbial populations, their physiological heterogeneities, and their local interactions with competing flora or antimicrobial agents. These specificities render CLSM particularly suitable for the analysis of pathogen dynamics throughout the food chain and as an essential tool for the development of new control strategies.