Transient overconsumption induced by a Western diet in rats is essential to the control of metabolic endotoxemia via regulation of LPS intestinal translocation
Résumé
Increased plasma level of gut bacteria-derived lipopolysaccharides (metabolic endotoxemia) due to alteration of intestinal homeostasis plays an important role in obesity-related disorders. We hypothesized
that initial calorie overconsumption during Western diet feeding in rats alters intestinal homeostasis, leading to increased lipopolysaccharides (LPS) translocation. Rats were fed for 1 or 6 weeks either a control diet (C), a Western Diet (45% fat) ad libitum (WDal) or at the same caloric level than C rats (WDpf).
Adiposity and weight gain were similarly increased in both groups of WD rats, whereas endotoxemia was increased only in rats fed WD without calorie overconsumption. WD feeding increased intestinal LPS detoxification mechanisms (alkaline phosphate activity) and transiently decreased caecal bile acids receptors mRNA, irrespective of the energy intake level. However, sustained increased in intestinal LPS permeability and decreased caecal pxr, md2 and myd88 mRNA level were observed in WDpf rats as opposed to transient effects at week 1 in WDal rats. A Western diet has both profound and transient effects on the mechanisms preventing LPS intestinal translocation. The transient effects seem to be driven by initial calorie intake and involved in the control of metabolic endotoxemia.
Domaines
Alimentation et NutritionOrigine | Fichiers éditeurs autorisés sur une archive ouverte |
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