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Communication Dans Un Congrès Année : 2011

Non-invasive SFG spectroscopy: a tool to reveal the conformational change of grafted chains due to bacterial adhesion

Résumé

In many fields such as biomedical or food industry, surface colonization by micro-organisms leads to biofilms formation that are tridimentional biostructures highly resistant to the action of antimicrobials, by mechanisms still unclear. In order to deepen our understanding of the initial interaction of bacteria cells with a solid surface, we analyze by in situ vibrational Sum Frequency Generation (SFG) spectroscopy the effect of the adhesion of hydrophilic Lactoccocus lactis bacteria and its hydrophobic mutants in distilled water on a self-assembled monolayer (SAM) of octadecanethiol (ODT) on a gold film. When a homogeneous bacterial monolayer is deposited on this ordered surface, SFG spectrum of the ODT SAM shows significant intensity changes from that in air or in water. Its modelling as a function of conformation allows to distinguish optical effects due to the water solution surrounding bacteria from conformational changes of the ODT SAM due to the presence of the bacteria cells. Futhermore, bacterial adhesion induces different measurable effects on the ODT SAM conformation, depending on the hydrophobic / hydrophilic character of the bacterial surface. Such a result deserves to be taken into account for the design of new materials with improved properties or to control biofilm formation.
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Dates et versions

hal-01594373 , version 1 (26-09-2017)

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Emilie Bulard, Henri Dubost, Marie-Pierre Fontaine-Aupart, Wanquan Zheng, Jean-Marie Herry, et al.. Non-invasive SFG spectroscopy: a tool to reveal the conformational change of grafted chains due to bacterial adhesion. SPIE/OSA European Conferences on Biomedical Optics, May 2011, Munich, Germany. ⟨10.1117/12.888786⟩. ⟨hal-01594373⟩
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