Perinatal antibiotic treatment of sows affects intestinal barrier and immune system in offspring - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2013

Perinatal antibiotic treatment of sows affects intestinal barrier and immune system in offspring

Résumé

Maternal environment during pregnancy and lactation influences health of the offspring and its microbiota. Immune tolerance to dietary and commensal microbiota antigens develops early in life. Our aim was to investigate intestinal barrier and local immune response to LPS in piglets deriving from sows whose microbiota was manipulated by antibiotics. Long-term consequences on gut adaptation to a high fat diet were examined in growing offspring. Sows were given amoxicillin (AMX; 40 mg/kg/d) per os (n=11 AMX) or not (n=12 CTL) from 10 d before to 21 d after parturition. One piglet per litter was sacrificed at post-natal day (PND) 14, 21 and after weaning at PND42. The remaining ones were given either a low fat (LF) or a high fat (HF) diet (to enhance LPS epithelial passage) from PND140 to 169. At PND14, ileal permeability was greater in AMX than CTL piglets (P<0.05). At PND21, no increase in proliferation of lamina propria mononuclear cells (LPMCs) in response to LPS was noticed, demonstrating LPMC tolerance in both groups. Analysis of laser-captured enterocyte transcriptome indicated specific changes in immune response pathways. Especially differential expression of BFIFB1, HPSE and FSTL4 genes demonstrated stimulation of proinflammatory activity, corroborating the increased TNFα secretion in response to LPS observed in LPMCs of AMX piglets. At PND42 gene analysis suggested a repression of the immune response towards weaning-induced stress in AMX piglets. At PND169, HF diet increased ileal permeability in CTL offspring only. By contrast, ileal explant sensitivity to LPS was altered in HF pigs, especially in AMX-HF pigs. Manipulating maternal microbiota modified offspring ileal barrier and immune response to LPS during the neonatal period. It also influenced the intestinal adaptive response to a HF diet in adult offspring, suggesting microbiota-related imprinting.
Fichier non déposé

Dates et versions

hal-01594212 , version 1 (26-09-2017)

Identifiants

  • HAL Id : hal-01594212 , version 1
  • PRODINRA : 372073

Citer

Stéphanie Ferret-Bernard, Gaëlle Boudry, Laurence Le Normand, Véronique Rome, Gérard Savary, et al.. Perinatal antibiotic treatment of sows affects intestinal barrier and immune system in offspring. 64. Annual Meeting of the European Federation of Animal Science (EAAP), Aug 2013, Nantes, France. Wageningen Academic Publishers, Annual Meeting of the European Association for Animal Production, 19, 2013, Annual Meeting of the European Association for Animal Production. ⟨hal-01594212⟩

Collections

INRA INRAE
45 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More