Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins
Résumé
Methyl groups are very useful probes of structure, dynamics and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter we discuss the approach for labelling methyl groups in E. coli based protein expression, as exemplified with the mitochondrial carrier GGC.
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