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Communication Dans Un Congrès Année : 2016

Extractive bioconversion of 3-hydroxypropionic acid : insights in extraction and mass transfer mechanisms

Résumé

3-hydroxypropionic acid (3-HP) is a key building-block to obtain from biomass since it can lead to acrylic acid, acrylates and new bio-based polymers. The DoE ranked it among the 12 "Top value-added chemicals from biomass"[1]. It can be synthesized through the bioconversion of biomass-derived substrates such as glycerol and sugars. Yields limitations due to strong inhibitions from the intermediate metabolite and the acidic product[2] make the implementation of an in-situ product recovery necessary which would allow the continuous extraction of 3-HP and the removal of inhibition effects. Such a process can be implemented through reactive liquid-liquid extraction assisted by a membrane contactor which makes the interface stable and prevents the direct contact of the microorganisms with the organic phase. Short chain organic acids being hydrophilic, an extractant must be used in the organic phase to trap such molecules in rather hydrophobic complexes. These extractants can consist in aliphatic amines able to interact selectively with acids. Better extraction performances are obtained when these amines are used in alcohols. Previous results have demonstrated that bioconversion media decrease the extraction yield and kinetics[3]. The aim of this study is to get insights into the specific role of bioconversion media on the highlighted limitations. First, in depth investigations of the extraction mechanisms of pure 3-HP in aqueous solutions led us to the elaboration of an original chemical model able to predict equilibrium states from a wide range of initial conditions. These results are used in comparison with other model media aiming at imitating several aspects of the real bioconversion media such as the pH, the 3-HP concentration, the presence of buffer, salts and organic molecules. The importance of each effect on the membrane assisted extraction performances are evaluated. Results showed that the presence of the solvent is necessary to reach good extraction performances. Given that the interactions between amines and 3-HP is of acid-base type, the pH of the bioconversion media is very important. The impact of anions on the recovery process is also highlighted and the phosphates from buffer or salts from pH regulation solutions have a great impact on the extraction efficiency. However, organic molecules such as metabolites, substrates, by-products or biomolecules exhibited less effects on the extraction. The results tend to show that the decrease in the extraction yields controls the decrease in the extraction kinetics. These findings are valuable for the optimization of an integrated process of extractive bioconversion.
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Dates et versions

hal-01567209 , version 1 (17-10-2022)

Identifiants

  • HAL Id : hal-01567209 , version 1
  • PRODINRA : 396109

Citer

Florian Chemarin, Marwen Moussa, Grégoire Burgé, Florent Allais, Ioan-Cristian Trelea, et al.. Extractive bioconversion of 3-hydroxypropionic acid : insights in extraction and mass transfer mechanisms. 6. International Conference on Engineering for Waste and Biomass Valorisation WasteEng2016., May 2016, Albi, France. ⟨hal-01567209⟩
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