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Article Dans Une Revue Journal de Pharmacie de Belgique Année : 1998

Detection of cationic phenolic derivatives at a surfactant modified screen-printed electrode and its promising application in enzyme immunoassay

B. Limoges
  • Fonction : Auteur
B. Schöllhorn
L. Authier
C. Degrand
  • Fonction : Auteur

Résumé

In a recent work,1 we have shown that the enzyme-label alkaline phosphatase commonly used in immunoassay, can be sensitively determined through the catalytic transformation of an anionic substrate (aromatic monoester phosphate) into a cationic electroactive product P+ (phenol derivated by a cationic function) which is thus selectively and sensitively detected at a screen-printed electrode coated with an anionic polymer film. In order to improve the technique, a new cation-exchanger-modified screen-printed electrode has been developed and its analytical performance is evaluated with several cationic phenol products. The modified screen-printed electrodes are prepared from a carbon ink loaded with the sodium salt of dodecylbenzene sulfonic acid. The anionic surfactant is strongly retained within the dry ink and it allows the accumulation of low concentrations (nanomolar) of cationic phenol derivatives to occur through an ion-exchange process at the electrode surface. The accumulated phenol compounds are detected by the voltammetric oxidation of their phenolic function. Some compounds are preferentially accumulated and detected depending on their affinity for the anionic ink. The presence of a large excess of substrate (monoesterphosphate of the product) does not prevent the accumulation of the corresponding product. Preliminary results obtained with alkaline phosphatase appear promising for applications in enzyme immunoassay.

Domaines

Chimie
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Dates et versions

hal-01561644 , version 1 (13-07-2017)

Identifiants

  • HAL Id : hal-01561644 , version 1

Citer

B. Limoges, B. Schöllhorn, L. Authier, C. Degrand. Detection of cationic phenolic derivatives at a surfactant modified screen-printed electrode and its promising application in enzyme immunoassay. Journal de Pharmacie de Belgique, 1998, 53 (3), pp.214. ⟨hal-01561644⟩
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