Ion-exchange voltammetry at a surfactant-doped electrode: Model of mass transfer kinetics to an anionic surface-charged electrode and its application for the sensitive determination of alkaline phosphatase - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Electroanalytical Chemistry Année : 2000

Ion-exchange voltammetry at a surfactant-doped electrode: Model of mass transfer kinetics to an anionic surface-charged electrode and its application for the sensitive determination of alkaline phosphatase

L. Authier
B. Schöllhorn
J. Moiroux
  • Fonction : Auteur
B. Limoges
  • Fonction : Auteur

Résumé

A theoretical model of mass transfer kinetics was developed for investigating the accumulation of cationic compounds at an anionic surfactant-doped screen-printed electrode. It was extended to an enzyme-generated cationic product for the indirect determination of alkaline phosphatase (AP). The model takes into account the analyte depletion, the kinetics of the ion-exchange reaction-diffusion and the kinetics of the enzymatic reaction. The relationship between the ion-exchange voltammetric anodic peak current and the enzyme incubation/accumulation time for a given concentration of AP was established and the validity of the theoretical model was verified experimentally using a new AP substrate/product couple. It was shown that the theoretical and experimental data are in agreement. The combination of the catalytic enzyme generation of the cationic product with its simultaneous preconcentration at a cation-exchanger-doped electrode allows one to envision the indirect determination of AP at femtomolar levels.

Domaines

Chimie

Dates et versions

hal-01561643 , version 1 (13-07-2017)

Identifiants

Citer

L. Authier, B. Schöllhorn, J. Moiroux, B. Limoges. Ion-exchange voltammetry at a surfactant-doped electrode: Model of mass transfer kinetics to an anionic surface-charged electrode and its application for the sensitive determination of alkaline phosphatase. Journal of Electroanalytical Chemistry, 2000, 488 (1), pp.48-58. ⟨10.1016/S0022-0728(00)00187-X⟩. ⟨hal-01561643⟩
6 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More