NanoHPLC with parallel electrospray and inductively coupled plasma MS detection for biomolecules analysis.
Résumé
The inductively coupled plasma mass spectrometer (ICP-MS) is an element and isotope specific technique which provides detection limits at the femtogram level regardless of the mol. structure of the analyte and its coelution with matrix components and interfering compds. These features make ICP-MS an ideal complementary detection technique to electrospray MS/MS in HPLC of metal complexes or biomols. contg. in their structure a heteroatom, such as, for example, sulfur, phosphorous, selenium or iodine. This article discusses two exptl. setups for interfacing nanoHPLC to ICP-MS and highlights potential added value of ICP-MS for quant. peptides mapping and proteins characterization. [on SciFinder(R)]