Rapid analysis for cadmium metallothionein complexes by HPLC using microparticulate stationary phases
Résumé
Different columns with microparticulate (1.5 and 2 μm) stationary phases were investigated for the analysis of the polymorphism of mammalian metallothionein (MT) by reversed-phase HPLC. When a non-porous 1.5 μm stationary phase was used, the duration of the chromatographic run was reduced 10-fold (in comparison with the conventional 5 μm packing) without any loss in resolution. The method was applied to the analysis of MT-1 and MT-2 preparations from rabbit liver. © Springer-Verlag 1999.