Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Development (Cambridge, England) Année : 2017

Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging

Ulla Saarela
  • Fonction : Auteur
Saad Ullah Akram
  • Fonction : Auteur
Audrey Desgrange
Aleksandra Rak-Raszewska
  • Fonction : Auteur
Jingdong Shan
  • Fonction : Auteur
Veli-Pekka Ronkainen
  • Fonction : Auteur
Janne Heikkila
  • Fonction : Auteur
Ilya Skovorodkin
  • Fonction : Auteur
Seppo J. Vainio
  • Fonction : Auteur

Résumé

Tissue, organ and organoid cultures provide suitable models for developmental studies, but our understanding of how the organs are assembled at the single-cell level still remains unclear. We describe here a novel fixed z-direction (FiZD) culture setup that permits high-resolution confocal imaging of organoids and embryonic tissues. In a FiZD culture a permeable membrane compresses the tissues onto a glass coverslip and the spacers adjust the thickness, enabling the tissue to grow for up to 12 days. Thus, the kidney rudiment and the organoids can adjust to the limited z-directional space and yet advance the process of kidney morphogenesis, enabling long-term time-lapse and high-resolution confocal imaging. As the data quality achieved was sufficient for computer-assisted cell segmentation and analysis, the method can be used for studying morphogenesis ex vivo at the level of the single constituent cells of a complex mammalian organogenesis model system.

Dates et versions

hal-01539793 , version 1 (15-06-2017)

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Citer

Ulla Saarela, Saad Ullah Akram, Audrey Desgrange, Aleksandra Rak-Raszewska, Jingdong Shan, et al.. Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging. Development (Cambridge, England), 2017, 144 (6, SI), pp.1113-1117. ⟨10.1242/dev.142950⟩. ⟨hal-01539793⟩
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