Regulation of appressorium-expressed pathogenicity genes in the anthracnose fungus Colletotrichum higginsianum - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2015

Regulation of appressorium-expressed pathogenicity genes in the anthracnose fungus Colletotrichum higginsianum

Résumé

Colletotrichum fungi cause devastating anthracnose diseases on numerous major crop plants worldwide. C. higginsianum attacks cultivated members of the Brassicaceae as well as the model plant Arabidopsis. Infection involves the differentiation of a series of specialized celltypes associated with host penetration (appressoria), growth inside living host cells(biotrophic hyphae) and tissue destruction (necrotrophic hyphae). Genome-wide expressionprofiling revealed waves of gene activation linked to each pathogenic transition. Remarkably,although appressoria formed in vitro are morphologically indistinguishable from those formedin planta, comparison of their transcriptomes showed over 1,500 genes are induced only upon host contact. These include genes encoding 120 enzymes of secondary metabolism, 64 secreted effector proteins and 81 enzymes potentially degrading plant cell wall polymers. To investigate how this coordinated expression of pathogenicity-related genes is orchestrated we are considering potential regulation at two levels: remodelling of chromatin structure and regulation by global transcriptional activators. For genetic manipulation we have targeted C. higginsianum orthologs of four well-characterized regulators of chromatin structure (DIM5, HP1, CclA and Kmt6), as well as the transcription factors SGE1 and ZIP1. Progress in the phenotypic characterization of these mutants will be presented
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Dates et versions

hal-01536581 , version 1 (11-06-2017)

Identifiants

  • HAL Id : hal-01536581 , version 1
  • PRODINRA : 368514

Citer

Richard O'Connell. Regulation of appressorium-expressed pathogenicity genes in the anthracnose fungus Colletotrichum higginsianum. mini-symposium “Epigenetics meets biotrophy”, Oct 2015, Aachen, Germany. ⟨hal-01536581⟩
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