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Communication Dans Un Congrès Année : 2015

Localization of Colletotrichum higginsianum putative effector proteins in planta

Résumé

Colletotrichum higginsianum causes anthracnose disease on cruciferous plants, including Arabidopsis. The fungus uses a hemibiotrophic infection strategy, involving the formation of several specialized cell types. After melanized appressoria puncture host surfaces, bulbous biotrophic hyphae develop inside living host cells, surrounded by a modified host plasma membrane; finally the fungus switches to destructive necrotrophy, associated with thin filamentous hyphae. The C. higginsianum genome encodes 365 putative effectors (ChECs), defined as predicted secreted proteins either with no homology to proteins outside the genus or resembling known effectors from other pathogens. Deep-sequencing of the C. higginsianum transcriptome during infection (Kleemann et al. 2012) and genome-wide expression analysis (O’Connell et al. 2012) revealed a set of 97 plant-induced effectors that are expressed in appressoria before host penetration and/or in biotrophic hyphae after penetration. This project aims to understand the role of these effectors in the early establishment of infection. Clues to effector function can come from knowing their destination inside host cells. We used Agrobacterium tumefaciens to transiently express 65 ChECs as N-terminal fusions with GFP in Nicotiana benthamiana leaf cells, revealing different patterns of subcellular localization. Nine ChECs are targeted to the plant nucleus, among which 3 specifically accumulate in the nucleolus, while 7 other ChECs localize to plant microtubules or organelles. To characterize effector functions, selected candidates are being expressed in transgenic Arabidopsis plants under 35S or dexamethasone-inducible promoters to investigate effects on plant immune responses, and their contribution to fungal virulence will be tested by targeted gene disruption in C. higginsianum.
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Dates et versions

hal-01536431 , version 1 (11-06-2017)

Identifiants

  • HAL Id : hal-01536431 , version 1
  • PRODINRA : 294765

Citer

Guillaume Robin, Richard O'Connell. Localization of Colletotrichum higginsianum putative effector proteins in planta. 28. Fungal Genetics Conference, Mar 2015, Asilomar, United States. ⟨hal-01536431⟩
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