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Communication Dans Un Congrès Année : 2015

Epigenetic hotspots are genomic islands for putative effector-encoding genes in Zymoseptoria tritici.

Résumé

The Dothideomycete Zymoseptoria tritici (synonym Mycosphaerella graminicola) is a pathogen of wheat (Triticum aestivum) and has a hemibiotrophic life style suggesting a fine-tuned regulation of gene expression. The genome of Z. tritici comprises 21 chromosomes including up to eight conditionally dispensable chromosomes (CDCs) containing a considerably higher proportion of transposable elements (TEs) than core chromosomes (Goodwin et al., 2011). Evidence is accumulating that genes involved in host-pathogen interaction, such as effector-genes, can be located in TE-rich, heterochromatic genomic regions. In Z. tritici, while CDCs are TE-rich, they harbor few effector-genes. We however hypothesized that effector-genes are not randomly located on core chromosomes. In order to investigate their location with respect to the chromatin structure, a genome-wide histone map was established using ChIP-sequencing and correlated with TE, effector-gene location and transcriptomic data. As expected, CDCs are mainly heterochromatic while gene-rich core chromosomes are mainly euchromatic, which is consistent with the low expression of genes located on CDCs compared to core chromosomes. TE-rich regions of the core chromosomes are also enriched in histone modifications typical of heterochromatic domains, suggesting that an epigenetic control of expression of the genes located in these regions could occur. We define these regions of the core chromosomes as “epigenetic hotspots”. Analysis of the location of effector-genes in relation to epigenetic hotspots highlighted that these genes are significantly associated with heterochromatic domains. We further investigated effect of this location on the regulation of expression of these pathogenicity-related encoding genes during two stages of wheat infection. We conclude that TE-rich regions of core chromosomes define genomic environments for putative effector-encoding genes and that histone modifications may control their expression.
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Dates et versions

hal-01535377 , version 1 (08-06-2017)

Identifiants

  • HAL Id : hal-01535377 , version 1
  • PRODINRA : 294434

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Jessica Louise Soyer, Jonathan Grandaubert, Klaas Schotanus, Janine Haueisen, Eva H Stukenbrock. Epigenetic hotspots are genomic islands for putative effector-encoding genes in Zymoseptoria tritici.. 28. Fungal Genetics Conference, Mar 2015, Asilomar, United States. ⟨hal-01535377⟩
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