A Tat menage a trois - The role of Bacillus subtilis TatAc in twin-arginine protein translocation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochimica et Biophysica Acta - Molecular Cell Research Année : 2015

A Tat menage a trois - The role of Bacillus subtilis TatAc in twin-arginine protein translocation

Résumé

The twin-arginine translocation system (Tat) is a protein transport system that moves fully folded and cofactor-containing proteins across membranes of bacteria, archaea and thylakoids. The minimal Tat pathway is composed of two subunits, TatA and TatC. In some organisms TatA has been duplicated and evolved to form a third specialized subunit, TatB. The Bacillus subtilis genome encodes two TatC subunits (TatCd and TatCy) and three TatA subunits (TatAd, TatAy and TatAc). These subunits combine to form two parallel minimal pathways, TatAy-TatCy and TatAd-TatCd. The purpose and role of the third TatA component, TatAc, has remained ambiguous. In this study we examined the translocation of two natively expressed TatAy-TatCy-dependent substrates, EfeB and QcrA, in various Tat-deficient genetic backgrounds. More specifically, we examined the ability of different mutated TatAy subunits to complement for the absence of wild-type TatAy. We further detailed a graded growth phenotype associated with the functional translocation of EfeB. We found that in various instances where specific amino add substitutions were made in TatAy, a definite TatAc-associated growth phenotype occurred in genetic backgrounds lacking TatAc. Altogether, our findings show that TatAy and TatAc interact and that this TatAy-TatAc interaction, although not essential, supports the translocation of the Tat substrate EfeB when TatAy function is compromised. This implies that the third TatA-like protein in B. subtilis could represent an intermediate evolutionary step in TatA-TatB specialization.

Dates et versions

hal-01535287 , version 1 (08-06-2017)

Identifiants

Citer

Vivianne J. Goosens, Alba de San Eustaquio Campillo, Rut Carballido y Lopez, Jan Maarten van Dijl. A Tat menage a trois - The role of Bacillus subtilis TatAc in twin-arginine protein translocation. Biochimica et Biophysica Acta - Molecular Cell Research, 2015, 1853 (10), pp.2745-2753. ⟨10.1016/j.bbamcr.2015.07.022⟩. ⟨hal-01535287⟩
85 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More