Microbiological identification by surface-enhanced Raman spectroscopy - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Applied Spectroscopy Reviews Année : 2016

Microbiological identification by surface-enhanced Raman spectroscopy

Résumé

New methods for pathogens identification are of growing interest in clinical and food sectors. The challenge remains to develop rapid methods that are more simple, reliable, and specific. Surface-enhanced Raman spectroscopy (SERS) appears to be a promising tool to compete with current untargeted identification methods. This article presents the intensive research devoted to the use of SERS for bacterial identification, from the first to the very recent published results. Compared to normal Raman spectroscopy, the introduction of nanoparticles for SERS acquisition introduces a new degree of complexity. Bacterial Raman fingerprints, which are already subject to high spectral variability for a given strain, become then very dependent on numerous experimental parameters. To overcome these limitations, several approaches have been proposed to prepare the sample, from the microbiological culture conditions to the analysis of the spectrum including the coupling of nanoparticles on the bacterial membrane. Main strategies proposed over the last 20 years are examined here and discussed in the perspective of a protocol transfer towards industry.
Fichier non déposé

Dates et versions

hal-01533379 , version 1 (06-06-2017)

Identifiants

Citer

Romain Chauvet, Fabienne Lagarde, Thomas Charrier, Ali Assaf, Gerald Thouand, et al.. Microbiological identification by surface-enhanced Raman spectroscopy. Applied Spectroscopy Reviews, 2016, 52 (2), pp.123 - 144. ⟨10.1080/05704928.2016.1209760⟩. ⟨hal-01533379⟩
141 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More