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Article Dans Une Revue (Article De Synthèse) Journal of Virology Année : 2016

Mutated but not deleted ovine PrPCN-terminal polybasic region strongly interferes with prion propagation in transgenic mice

Manal Khalife
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Bruno Passet

Résumé

Mammalian prions are proteinaceous infectious agents composed of misfolded assemblies of the host-encoded, cellular prion protein (PrP). Physiologically, the 23-31 N-terminal polybasic region of PrP has been shown to be involved in its endocytic trafficking and interactions with glycosaminoglycans or putative ectodomains of membrane-associated proteins. Several recent reports also describe this PrP region as important for the toxicity of mutant prion proteins and the efficiency of prion propagation, both in vitro and in vivo. The question remains as to whether the latter observations made with mouse PrP and mouse prions would be relevant to other PrP species/prion strain combinations given the dramatic impact on prion susceptibility of minimal amino acid substitution and structural variations in PrP. Here, we report that transgenic mouse lines expressing ovine PrP deleted for residues 23-26 (KKRP) or mutated in this N-terminal region (KQHPH instead of KKRPK) exhibited a variable, strain-dependent susceptibility to prion infection with regard to the proportion of affected mice and disease tempo relative to their wild-type counterparts. Deletion has no major effect on 127S scrapie prion pathogenesis, whereas mutation increased by almost 3-fold the survival time of the mice. Deletion marginally affected the incubation time of scrapie LA19K and ovine BSE prions, whereas mutation caused apparent resistance to disease. IMPORTANCE Recent reports suggested that the N-terminal polybasic region of the prion protein could be a therapeutic target to prevent prion propagation or toxic signaling associated with more common neurodegenerative diseases such as Alzheimer's disease. Mutating or deleting this region in ovine PrP completes the data previously obtained with the mouse protein, by identifying the key amino acid residues involved.

Dates et versions

hal-01532513 , version 1 (02-06-2017)

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Manal Khalife, Fabienne Reine, Sophie Paquet, Johan Castille, Laetitia Herzog, et al.. Mutated but not deleted ovine PrPCN-terminal polybasic region strongly interferes with prion propagation in transgenic mice. Journal of Virology, 2016, 90 (3), pp.1638-1646. ⟨10.1128/JVI.02805-15⟩. ⟨hal-01532513⟩
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