Genetic and molecular basis of botrydial biosynthesis. Connecting cytochrome P450-encoding genes to biosynthetic intermediates. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue ACS Chemical Biology Année : 2016

Genetic and molecular basis of botrydial biosynthesis. Connecting cytochrome P450-encoding genes to biosynthetic intermediates.

Résumé

Over two hundred species of plants can be infected by the phytopathogenic fungus Botrytis cinerea under a range of different environmental conditions. In response to these the fungus produces unique terpenoid and polyketide metabolites. Parts of the plants may be killed by the phytotoxin, botrydial enabling the fungus to feed on the dead cells. In this paper we describe the genetic and molecular basis of botrydial biosynthesis and the function of the five genes of the genome of B. cinerea which together constitute the botrydial biosynthetic gene cluster. Genes BcBOT3 and BcBOT4, encoding two cytochrome P450 monooxygenases, were inactivated by homologous recombination and were shown to catalyse regio- and stereospecific hydroxylations, at the carbons C-10 and C-4 respectively, of the presilphiperfolan-8β-ol skeleton. The null mutants i.e. bcbot3Δ and bcbot4Δ accumulated key intermediates in the botrydial biosynthesis enabling the complete genetic and molecular basis of the botrydial biosynthetic pathway to be established. Furthermore, the bcbot4Δ mutant overproduced a significant number of polyketides, which included, in addition to known botcinins, botrylactones and cinbotolide A, two new botrylactones and two new cinbotolides, cinbotolides B and C.

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hal-01531674 , version 1 (31-01-2024)

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Javier Moraga, Berengere Dalmais, Inmaculada Izquierdo-Bueno, Josefina Aleu, James R. Hanson, et al.. Genetic and molecular basis of botrydial biosynthesis. Connecting cytochrome P450-encoding genes to biosynthetic intermediates.. ACS Chemical Biology, 2016, 11 (10), pp.2838-2846. ⟨10.1021/acschembio.6b00581⟩. ⟨hal-01531674⟩
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