Leptosphaeria maculans effectors involved in the oilseed rape systemic colonization. - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2017

Leptosphaeria maculans effectors involved in the oilseed rape systemic colonization.

Résumé

The stem canker disease, caused by Leptosphaeria maculans, is one of the most devastating diseases of oilseed rape (canola). It colonizes the plant in two stages: a short and early colonisation stage corresponding to cotyledon or leaf colonisation, and a late colonisation stage during which the fungus colonises systemically and symptomlessly the plant during several months before stem canker appears. To date, determinants of the late colonisation stage remain poorly understood. By a transcriptomic approach, we previously identified two waves of effector candidate expression during the early and late colonisation stages (Gervais et al, 2016). The late effector candidates are located in gene-rich genomic regions, whereas the early effector genes are located in gene-poor regions of the genome. Among the late effector candidates identified, we selected 6 genes for further characterization. We created mutants silenced for these effector candidates. For one of these genes, its expression level correlated negatively with the size of the necrosis observed in the stem. The identification of new effector genes would contribute to the identification of new resistance genes specific to these effectors. To easily identify matching resistance genes in oilseed rape, we created transgenic isolates expressing these 6 late effectors at the early steps of infection to provide medium-throughput strategies to screen more efficiently different cultivars. Preliminary results indicate that some cultivars with adult resistance were more resistant to these transgenic isolates in cotyledon assays. With this approach, we also identified a cultivar carrying a specific resistance to one these 6 effector candidates. Reference Gervais, J., Plissonneau, C., Linglin, J., Meyer, M., Labadie, K., Cruaud, C., Fudal, I., Rouxel, T. and Balesdent, M.H. (2016) Different waves of effector genes with contrasted genomic location are expressed by Leptosphaeria maculans during cotyledon and stem colonization of oilseed rape. Mol. Plant Pathol.
Fichier non déposé

Dates et versions

hal-01530817 , version 1 (31-05-2017)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

  • HAL Id : hal-01530817 , version 1
  • PRODINRA : 389962

Citer

Julie Gervais, Clémence Plissonneau, Juliette Linglin, Michel Meyer, K Labadie, et al.. Leptosphaeria maculans effectors involved in the oilseed rape systemic colonization.. 29. Fungal genetics conference Asilomar 17, Mar 2017, Pacific Grove CA, United States. p.234. ⟨hal-01530817⟩
165 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More